NEWPORT 优铂特 OD8-DCL498-D ODiate® Fluorescence Dichroic Beamsplitters
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NEWPORT 优铂特 OD8-DCL498-D ODiate® Fluorescence Dichroic Beamsplitters
ODiate® Dichroic Beamsplitter, 25.2 x 35.6 mm, 498 nm 50% Cut-on Edge Wavelength
The OD8-DCL498-D Dichroic Beamsplitter is specified to reflect the excitation and transmit the emission of GFP and supports similar alternative fluorophores, designed in conjunction with OD8-EX474-37-B and OD8-EM523-41-B . The filter is manufactured to provide maximum signal and sensitivity delivering exceptionally high excitation passband refleciton ≥ 98% avg and emission passband transmission ≥ 96% avg for a 498 nm 50% Cut-on edge wavelength.
Alternate Fluorophores
In addition to GFB the following fluorophores are also compatible with this set:
5-FAM, Abberior STAR GREEN, Azami Green, Azami Green (monomeric), BCECF@pH5.2, BCECF@pH5.5, C3-Oxacyanine, Calcein, CF 450, CF 488A, CFP2, Coumarin 6, Cy®2, DiO, Emerald, FITC, Fluo-4, Fluorescein (0.1M NaOH), Fluorescein (IgG), HCS CellMask Green, LysoSensor Green, mAzamiGreen, mEmerald, mHoneydew, MitoSpy Green FM, MitoTracker® Green FM, mWasabi, NBD, NBD-X, NeuroTrace® 500/525 Green Fluorescent Nissl Stain (RNA), PromoFluor-488, PromoFluor-488 Premium, PS-CFP2, sgGFP, SYBR® Green I, SYTO® 13, SYTO® 16, SYTO® 9, TagGFP, TagGFP2, TurboGFP, WEGFP (post-activation), YO-PRO®-1, YOYO®-1.
Optimized Coatings and Engineering Expertise
Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance. Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries.
Environmental Stability and Physical Durability
The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.
参数
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类型Fluorescence Dichroic Beamsplitter
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起始/截止波长498 nm (50%)
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反射波段≥98% avg, 458-491.5 nm
≥98% avg, 350-408.5 nm -
透射带≥96% avg, 419-479.5 nm
≥95% avg, 419-900 nm -
透射光束偏移≤ 1 wave PV @ 632.8 nm
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入射角45°±1.5°
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Cone Half-angle< 2°
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材料Fused Silica
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尺寸25.2 x 35.6 mm
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尺寸公差±0.1 mm
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厚度1.0 mm
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厚度公差±0.1 mm
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表面质量60-40 scratch-dig
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平行度≤ 5 arcsecond
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荧光团GFP
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防潮性能Per MIL-STD-810E, method 507.3, procedure III, modified to 5 cycles
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工作温度-50 to 100°C
清洁See How to Clean Optics
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